Determination of transgene stability in Nannochloropsis sp. transformed with immunogenic peptide for oral vaccination against vibriosis
نویسندگان
چکیده
Abstract Vibriosis is one of the common diseases caused by gram-negative bacteria from genus Vibrio . To treat vibriosis, vaccination has been proven to be most effective treatment as it can avoid risk drugs or antibiotics resistance. Microalgae are commonly used feed for aquatic organisms and Nannochloropsis sp. highly utilized species fish feed. This study focused on use microalga, a vaccine carrier. Transgenic harbouring an outer membrane protein kinase ( OmpK ) gene fragment namely V1, V2, CV1, CV2, CPV1 CPV2 were in this study. The stability transgenic over number generations was evaluated. DNA RNA lines extracted subjected PCR amplification fragment. successfully amplified expressed up fifth generation (F5). For CV1 present fourth (F4) F5 respectively but genes F4. From results obtained, shown suitable carrier ameliorate vibriosis.
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ژورنال
عنوان ژورنال: Aquaculture International
سال: 2021
ISSN: ['1573-143X', '0967-6120']
DOI: https://doi.org/10.1007/s10499-020-00634-w